The T-DNA integration characteristics of monokaryotic mutant library in Lentinula edodes

Chen Wang,Haiying Zhong,Xiaolian Long,Ruiping Xu,Yuhua Gong,Yinbing Bian,Yan Zhou

Scientia Horticulturae（2023）

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摘要

•First establishment of Lentinula edodes T-DNA insertional monokaryotic mutant library.•Proposed the insertion and integration mode of foreign T-DNA in L. edodes.•Screen out some T-DNA insertional mutants with altered thermotolerance can be used for further study on gene function of L. edodes.

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关键词

Lentinula edodes,Mutant library,T-DNA integration,Gene function,Resequencing

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