Fish oil supplementation protects against protein undernutrition-induced testicular and ovarian biochemical alterations in rats.

Proteins are required for biological functions and their inadequacy might impair the growth and development of the reproductive system. The study investigated the effects of fish oil (FO) supplementation on low-protein diet-induced alterations in male and female reproductive organs. Male and female rats were assigned randomly to four groups respectively. The NPD rats had five rats per group and were given 16% casein diet while the LPD rats had eight rats per group and received 5% casein diet. After the 8th week, FO was administered for 3 weeks via oral gavage at a concentration of 400 mg Kg after which the rats were sacrificed and testes and ovaries were excised. LPD-fed rats showed lower body weights for both genders. In LPD-fed rats, NO was significantly increased while GSH, vitamins C and E levels, the activities of CAT (except in ovaries), and GST were significantly reduced in both tissues. The activities of SOD and GPx were only reduced in the testes including sperm count, motility, and increase deformed sperm cells. Testosterone and progesterone levels were also reduced and lipid homeostasis was disrupted in the plasma of LPD-fed rats. FO supplementation reduces the NO, CHOL, TG, LDL (in females), and VLDL but significantly improves HDL (in females), testosterone, and progesterone levels, sperm count, motility, and morphology. The antioxidant status of both tissues also increased significantly in LPD-fed rats. Conclusively, FO might be effective in improving testicular and ovarian functions and for the maintenance of plasma lipid homeostasis in LPD-fed rats.