青风藤叶枯病病原菌的分离鉴定及防治药剂的筛选
wf(2023)
Abstract
2021年春季,从湖南省怀化市中方县正清制药青风藤基地和湖南农业大学试验基地分别采集青风藤叶枯病病叶,利用组织分离法获得病原菌,并进行柯赫氏法则验证.借助形态学鉴定和分子系统学分析,明确青风藤叶枯病病原为交链隔孢(Alternaria alternata).利用菌丝生长速率法对5种药剂(95%苯醚甲环唑、98%咪鲜胺、96%己唑醇、96.5%丙硫菌唑、98%嘧菌酯)进行筛选,结果显示95%苯醚甲环唑抑菌效果最好,EC50为0.0436 mg/L;98%嘧菌酯抑菌效果最差,EC50值为8.4473 mg/L.
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Key words
Caulis sinomenii,leaf blight,isolation and identification of the pathogen,Alternaria alternata,screening of fungicides
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