免疫亲和柱净化-液相色谱-串联质谱法同时测定水产品中8种霉菌毒素

Science and Technology of Food Industry(2023)

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Abstract
目的:建立免疫亲和柱净化-液相色谱-串联质谱法(LC-MS/MS)同时测定水产品中8种霉菌毒素残留.方法:样品经乙腈水溶液(84:16,V:V)提取,多毒素免疫亲和柱净化.色谱柱为Agilent Proshell 120 SB·C18(2.1 mm×100 mm,2.7μm),流动相为甲醇-5 mmol/L乙酸铵(含0.1%甲酸),梯度洗脱,流速为0.4 mL/min,柱温为40℃.采用电喷雾离子源正负离子同时扫描,多反应监测模式(MRM)进行质谱检测.结果:8种霉菌毒素在1.0~50.0 ng/mL范围内线性关系良好(R2>0.992),方法检出限在0.05~0.50μg/kg之间,定量限在0.17~1.65μg/kg之间;8种霉菌毒素加标回收率在75.6%~106.3%之间,相对标准偏差(RSD)为3.5%~9.3%.结论:该方法操作便捷、灵敏度高、准确可靠,能满足水产品中多种霉菌毒素残留快速检测需求.
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Key words
mycotoxins,aquatic products,liquid chromatography-tandem mass spectrometry,immunoaffinity column
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