Detection of Mycoplasma species at various anatomical sites of dogs from different types of kennels

This work aimed to detect Mycoplasma cynos , M. canis , M. edwardii , and M. molare in different types of kennels, in addition to evaluating their distribution in different colonization sites. The dogs belonged to different kennels from armed forces ( n = 3), shelters ( n = 3), and commercial purposes ( n = 2). Samples of the oropharynx, genital mucosa, and ear canal were collected from each dog ( n = 98), totaling 294 samples. Aliquots were submitted to isolation and the samples confirmed as Mycoplasma spp. were subjected to conventional PCR for M. canis and multiplex PCR for M. edwardii , M. molare , and M. cynos detection. Of the 98 dogs studied, 63.3% (62) were positive in at least one anatomical site evaluated for Mycoplasma spp. Among the 111 anatomical sites positive for Mycoplasma spp., M. canis , M. edwardii , and M. molare were detected in 29.7% (33/111), 40.5% (45/111), and 2.70% (3/111), respectively. No animal was positive for M. cynos.