Novel Lipase from Golden Pompano ( Trachinotus ovatus ) Viscera: Purification, Characterization, and Application in the Concentrating of n -3 Polyunsaturated Fatty Acids

Lipases have been widely applied in a variety of industrial fields, such as food, pharmaceuticals, biofuels, and biotechnology. Recent years have witnessed a great interest in modifying lipids for the production of triacylglycerols enriched with n -3 polyunsaturated fatty acids (PUFAs). Here, a novel salt-tolerant, organic solvent-stable, and bile salt-activated lipase was purified from golden pompano ( Trachinotus ovatus ) viscera, which was named as golden pompano lipase (GPL). GPL had a specific activity of 57.2 Umg −1 with an estimated molecular weight of 14 kDa, exhibited optimal activity at 40°C and pH 8.0, and showed K m and V max of 40.16 µmolL −1 and 769.23 µmolL −1 min −1 , respectively. GPL activity was enhanced by Mn 2+ and sodium deoxycholate. It was active in organic solvents, including methanol, ethanol, chloroform, and hexane. GPL also showed a good salinity tolerance of up to 1 molL −1 . n -3 PUFA enrichment in the glyceride fraction of golden pompano oil was performed by GPL-catalyzed hydrolysis and yielded a total PUFA concentration of 56.99%. EPA, DHA, and DPA were enriched by 10.4-, 3.2-, and 1.8-fold of their initial levels, respectively. This study recognized the industrial applicability of GPL to prepare enriched C 20−22 n -3 PUFA.