Hypoglycemic effect of Coffea arabica leaf extracts and major bioactive constituents.

The present study focused on investigating the antioxidant, antiglycation activity, digestive enzymes inhibition, bioaccessibility and hypoglycemic effect of leaves extracts. The extracts deactivated the O•, ROO•, HO, HOCl reactive oxygen species. Coffee leaves showed strong inhibition of α-glucosidase (IC = 40.30 μg mL) greater than the isolated metabolites and acarbose. There was also inhibition of pancreatic lipase (IC = 56.43 μg mL) in addition to a hypoglycemic effect in zebrafish similar to acarbose and metformin. With the exception of rutin, all biocompounds were detected at all stages of digestion. Finally, these results suggest that leaf extracts possess antidiabetic and anti-obesity properties that can be attributed to the main metabolites and the synergistic action between them.Communicated by Ramaswamy H. Sarma.