Near-infrared enhances antiangiogenic potentiality of quinacrine-gold hybrid nanoparticles in breast cancer stem cells via deregulation of HSP-70/TGF-beta

Aim: This study aimed to explore the antiangiogenic mechanism of quinacrine-gold hybrid nanoparticle (QAuNP) and near-infrared (NIR) radiation in patient-derived primary breast cancer stem cells. Materials & methods: Various cell-based in ovo angiogenesis and in vivo patient-derived xenograft mouse systems were used as models for the study. Results: The experimental results showed that QAuNP + NIR treatment deregulated the HSP-70/TGF-beta physical interaction in primary breast cancer stem cells. Reduced TGF-beta secretion in the tumor microenvironment inhibited angiogenesis activation in endothelial cells by deregulating the TGF-beta-mediated PI3K/AKT/mTOR cascade. Conclusion: This study revealed that QAuNP + NIR irradiation downregulated HSP-70 expression, inhibited the HSP-70/TGF-beta interaction, reduced the secretion of TGF-beta in the tumor microenvironment and ultimately inhibited TGF-beta-mediated angiogenesis. [GRAPHICS] Plain language summary: This study discovered that the formation of blood vessels in breast cancer is significantly reduced when hybrid nanoparticles and infrared laser therapy are used to treat breast cancer stem cells. The secretory cytokines in the tumor microenvironment primarily responsible for developing blood vessels in the tumor are dramatically reduced by treatment. As a result, the tumor's blood vessel growth is reduced, making it difficult for the cancer cells to get the nutrients and oxygen they need to survive. Tweetable abstract: Near-infrared radiation enhances the antiangiogenic potentiality of quinacrine-gold hybrid nanoparticles in patient-derived breast cancer stem cells via deregulation of the HSP-70/TGF-beta axis.