Box-Behnken Designs for the Optimization of the Ethanol Extraction Process for Chuilian Jianpi Granules

Objective: The extraction process of Chuilian Jianpi granules was optimized.Methods: The extraction process of Chuilian Jianpi granules was optimized by box-Behnken response surface method, and the comprehensive score was calculated based on the content of geniposide in the extract and the yield of extract.Results: The optimal extraction process was as follows: add water volume multiple of 10 times, cook twice, cook for 3h each time. Under this condition, the comprehensive score was 96.816. The comprehensive score of the validation test was 99.07(RSD=0.54%, n=3), and the results were basically consistent.Conclusion: The process is stable and feasible, which can provide basis for the preparation of Chuilian Jianpi granules.