Fun30 nucleosome remodeller regulates white-to-opaque switching in Candida albicans .

( . ) is an opportunistic pathogen in humans and possesses a white-opaque heritable switching system. Wor1 is a master regulator of white-opaque switching and is essential for opaque cell formation in . . However, the regulatory network of Wor1 in white-opaque switching is still vague. In this study, we obtain a series of Wor1-interacting proteins using LexA-Wor1 as bait. Among these proteins, function unknown now 30 (Fun30) interacts with Wor1 and . Fun30 expression is upregulated in opaque cells at the transcriptional and protein levels. Loss of attenuates white-to-opaque switching, while ectopic expression of significantly increases white-to-opaque switching in an ATPase activity-dependent manner. Furthermore, upregulation is dependent on CO ; loss of , a key CO -sensing transcriptional regulator, abolishes upregulation. Interestingly, deletion of affects the expression regulation feedback loop. Thus, our results indicate that the chromatin remodeller Fun30 interacts with Wor1 and is required for expression and opaque cell formation.