Association of polymorphisms in exon 6 and 3 '-untranslated region of the OLR1 gene with milk production traits in Sahiwal cattle
JOURNAL OF APPLIED ANIMAL RESEARCH(2023)
摘要
The present investigation was undertaken to explain the association of SNPs (single nucleotide polymorphisms) in exon 6 and 3 '-UTR of OLR 1 gene with milk traits in Sahiwal cattle. Milk traits were observed from 328 Sahiwal cows sired by 64 bulls viz: - First lactation 305 days milk yield (Kg) (FL305MY), Average test day milk yield (Kg) (ATDMY), Average test day fat percentage (%) (ATDFP) and Lactational fat yield (Kg) (LFY). The animal model was used to estimate the breeding values for FL305MY and LFY. The traits ATDMY and ATDFP were corrected for significant non-genetic factors by using LSML-Harvey and subsequently used for association studies. A total of 130 animals for PCR-RFLP and 103 for sequencing were screened through three sets of primers for the presence of polymorphisms in exon 6 and 3 '-UTR of the OLR1 gene. The CC genotype of SNP C337A in the 3 '-UTR of OLR1 gene had a significant effect (p < 0.01) on ATDFP and LFY. Allele combination CGC had a positive effect (p < 0.05) on ATDFP. Identified SNP C337A and CCGGCC haplotype can be used as potential markers for improving milk yield and composition traits in Sahiwal cattle.
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关键词
SNP,PCR-RFLP,exon 6,OLR1 gene,Sahiwal
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