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Light-Seq: from microscopy to transcriptomics and back

Sinem K. Saka,Jocelyn Y. Kishi

NATURE METHODS（2022）

Cited 11|Views21
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Abstract
Light-Seq combines high resolution imaging with next generation sequencing of selected cell populations in fixed biological samples. Specifically, microscopically analyzed cells can be subjected to RNA expression profiling while keeping the sample intact for further assays, enabling cellular phenotypes and states to be assessed in the context of the original tissue.
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