Taxi Drivers' Response to Cancellations and No-Shows: New Evidence for Reference-Dependent Preferences

Microplastics (MPs) are emerging contaminants in aquatic environments, yet their impact on sediment microbiota and biogeochemical processes were not well reported. Herein, microcosm experiments were performed to investigate the effects of MPs (Polystyrene, PS and Polyethylene, PE) with three size classes (ranging from 100 nm to 150-200 mu m) on sediment bacterial and fungal communities over 60-day incubation from Taihu Lake. High-throughput sequencing revealed the alpha diversities of bacterial and fungal communities were reduced by MPs, dependent on MPs' size and type. Bacterial community structures were significantly altered under all MPs treatments, with clustering for the same size class for PS and PE. Fungal community structures were significantly affected for all MPs, with PS and PE exhibiting different effects. Co-occurrence network analysis suggested MPs changed bacterial and fungal network complexities. Proteobacteria and Ascomycota formed strong associations with other phyla and demonstrated tolerance to MPs exposure. Actinobacteria, Firmicutes, and Chytridiomycota were the main respondents to MPs. The enzyme concentrations were stimulated by MPs, indicating carbon and nitrogen uptakes might be increased. Therefore, PS and PE had similar impacts on the microbial community (particularly bacteria), and sizes of MPs were the main influencing factors. MPs shifted community structure and network with distinct responses from bacteria and fungi, likely leading to the alteration of microbial-involved carbon and nitrogen cycling.