Molecular Cloning and Functional Characterization of Galectin-1 in Yellow Drum ( Nibea albiflora ).
International journal of molecular sciences(2023)
摘要
Galectins are proteins that are involved in the innate immune response against pathogenic microorganisms. In the present study, the gene expression pattern of (named as ) and its function in mediating the defense response to bacterial attack were investigated. The tertiary structure of Gal-1 protein consists of homodimers and each subunit has one carbohydrate recognition domain. Quantitative RT-PCR analysis indicated that was ubiquitously distributed in all the detected tissues and highly expressed in the swim-bladder of , and its expression could be upregulated by the pathogenic attack in the brain. Expression of Gal-1 protein in HEK 293T cells was distributed in the cytoplasm as well as in the nucleus. The recombinant Gal-1 protein by prokaryotic expression could agglutinate red blood cells from rabbit, , and . The agglutination of red blood cells by the recombinant Gal-1 protein was inhibited by peptidoglycan, lactose, D-galactose, and lipopolysaccharide in certain concentrations. In addition, the recombinant Gal-1 protein agglutinated and killed some gram-negative bacteria including , , , , , and . These results set the stage for further studies of Gal-1 protein in the innate immunity of .
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关键词
Nibea albiflora,agglutination,antibacterial activity,galectin-1,innate immunity
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