Levilactobacillus yiduensis sp. nov., Isolated from Zha-chili in Yidu County, Hubei Province, China

The zha-chili isolate HBUAS62285 T is a facultative anaerobic strain. This bacterium was gram-positive, but it could not generate catalase, was not motile, did not form spores, had no flagella, and produced gamma-aminobutyric acid (GABA). Comparing of HBUAS62285 T with its related type strains Levilactobacillus suantsaiihabitans BCRC 81129 T , Levilactobacillus angrenensis M1530-1 T , Levilactobacillus cerevisiae DSM 100836 T , Levilactobacillus wangkuiensis 6-5(1) T , Levilactobacillus lanxiensis 13B17 T , and Levilactobacillus mulengensis 112-3 T showed that the similarity of 16S rRNA gene sequence was less than 99.13%. Strain HBUAS62285 T has a G+C content of 50.57 mol%, an ANI value of less than 86.61%, an AAI value of less than 92.9%, and a dDDH value of less than 32.9% when compared to the aforementioned closely related strains. In the end, the most major fatty acids in cells were determined to be C 16:0 , C 18:1 ω 9c, C 19:1 cyclo ω 9,10c, and summed feature 10. Overall, the results of the phenotypic, genomic, chemotaxonomic, and phylogenetic studies indicate that strains HBUAS62285 T and CD0817 constitute a new species within the genus Levilactobacillus , and the name Levilactobacillus yiduensis sp. nov. is proposed. The type strain is HBUAS62285 T (= JCM 35804 T = GDMCC 1.3507 T ).