5-End Mapping in Human Mitochondrial DNA

Methods in molecular biology (Clifton, N.J.)（2023）

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Abstract

Here, we describe an assay that enables mapping of 5 '-ends across the genome using next-generation sequencing on an Illumina platform, 5 '-End-sequencing (5 '-End-seq). We use this method to map free 5 '-ends in mtDNA isolated from fibroblasts. This method can be used to answer key questions regarding DNA integrity, DNA replication mechanisms and to identify priming events, primer processing, nick processing, and double strand break processing on the entire genome.

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Key words

5 '-End-seq,DNA replication,DNA polymerase,mtDNA,RNase H1,Next-generation sequencing,Bioinformatics,Genomics

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