How to prepare a trichogram with transparent enamel base coat?

The trichogram is an easy and semi-invasive method, useful for evaluating different types of hair loss in daily practice. To date, there is no standardized methodology on how to collect and perform the exam. The use of a liquid interface between the slide and the coverslip to read the trichogram under optical microscopy varies widely in the literature. Among the alternatives, transparent enamel base coat is an inexpensive, accessible, and practical option, providing the examiner with proper visualization of the hair shafts with minimal air bubbles formation.