Site-specific Labeling of Antibodies with Quantum Dots Could Promote to Retain the Antigen Binding Capacity of Antibodies.
Food chemistry(2023)
摘要
A major concern with antibody labeling is the decreased antigen affinity binding capacity of antibodies, owing mainly to the randomly oriented binding of the marker. Herein, a universal approach for site-specific photo-crosslinking of quantum dots (QDs) to the Fc-terminal of antibodies was investigated utilizing antibody Fc-terminal affinity proteins. Results showed that the QDs only bound to the heavy chain of the antibody. Further comparative tests confirmed that the site-specific directed labeling approach maximizes the retention of the antigen-binding capacity of the natural antibody. Compared with the commonly employed random orien-tation labeling approach, the directional labeling approach allows the labeled antibody showed 6 times greater binding affinity to antigen. QDs-labeled monoclonal antibodies were applied to fluorescent immunochromato-graphic test strips for the detection of shrimp tropomyosin (TM). The established procedure has a detection limit of 0.054 mu g/mL. Thus, the site-specific labeling approach significantly improves the antigen binding capacity of the labeled antibody.
更多查看译文
关键词
Site -specific,Labeling,Antigen -binding capacity,Quantum dots,Shrimp tropomyosin,Immunochromatographic test strips
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要