Application of different in vitro tests for the precise diagnosis of double sensitization to Vespula-Polistes.

Supplementary Figure 1.Basophil Activation Test.1a) Basophils were identified in whole blood as low SSC/CD123+/HLA-DR-(A,B). Dotplots of CD63 expression after in-vitro stimulation with PBS (C), anti-IgE antibody (D), Vespula spp.(5μg/mL; E) and Polistes dominula (5μg/mL; F).Activated basophils are expressed as the percentage of CD63+ cells.1b) Percentage of CD63 basophils after challenge with 1-5-10µg/ml of each venom.