Site-selective covalent immobilization of PPARγ using a label-free strategy for chromatographic study

Qingqing Yao,Jiahuan Chen,Xuechao Li,Wen Yang,Jianan Ning,Qi Liang,Qian Li

MICROCHEMICAL JOURNAL（2023）

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摘要

•Exploration of a label free protein immobilization strategy that prevents spatial restriction.•Investigation the interaction of the ligands that are noncompetitive to GW9662 with PPARγ.•Development of a chromatographic method for adaption to other covalent inhibitor-protein pairs.

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关键词

Site-selective,Protein immobilization,PPARγ,Label-free,GW9662

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