Nano and micro-vesicle diffusion through proteins, electrostatics mediate its interactions

The primary biochemical communication in the body is mediated by vesicles, from energy transport to neural signals. Understanding the driving forces of its diffusion will proportionate light to comprehend the nature of some diseases. In this work, we investigate the diffusion of nano and microvesicles in a bovine serum albumin solution, as we alter their temperature and electrostatic charge. Their molecular interactions were studied by differential scanning calorimetry and its short-long range interactions by dynamic light scattering and video tracking. Results showed that the phase transition of the lipids alters its diffusion drastically and that the nano-size vesicles with net negative electrostatic charge are the most reactive to the protein.