Tubulin-Based Polymorphism (TBP) in Plant Genotyping.

Methods in molecular biology (Clifton, N.J.)(2023)

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摘要
Tubulin-based polymorphism (TBP) is an intron length polymorphism (ILP) method widely applicable to any plant species and particularly suitable for a first and rapid classification of any plant genome. It is based on the selective, polymerase chain reaction (PCR)-based amplification of the two introns present at conserved positions within the coding sequences of plant β-tubulin genes. Amplification releases a simple yet distinctive genomic profile.
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关键词
DNA fingerprinting,DNA polymorphism,Introns,Molecular markers,Tubulin
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