Three novel Actinoplanes species isolated by using polyaspartic acid as a water-retaining agent for the enrichment in situ .

Three novel actinomycete strains, designated TRM66264-DLM, TRM88002 and TRM88003, were isolated by using polyaspartic acid as a water-retaining agent for the enrichment . The 16S rRNA gene sequence and phylogenetic analyses of three strains indicated that they belonged to the genus . The phylogenetically closest strains of TRM66264-DLM, TRM88002 and TRM88003 were LIPI11-2-Ac043 (98.4 %), A4029 (98.0 %) and IFO15555 (98.1 %), respectively. The major polar lipids of strains TRM66264-DLM and TRM88002 were phosphatidylethanolamine and disphosphatidylglycerol, while strain TRM88003 only had phosphatidylethanolamine. The predominant menaquinones of strain TRM66264-DLM were identified as MK-9(H) and MK-9 (H). Strains TRM88002 and TRM88003 had MK-9(H). The cell-wall peptidoglycan of three strains contained -diaminopimelic acid. The whole-cell sugars of strain TRM66264-DLM were identified as arabinose, glucose, galactose and xylose. Strains TRM88002 and TRM88003 mainly had arabinose and glucose. The DNA G+C content of strains TRM66264-DLM, TRM88002 and TRM88003 were 70.48, 70.46 and 70.64 mol%, respectively. Genotypic and phenotypic analysis confirmed that all three strains sre new members of the genus . Therefore, it is proposed that strains TRM66264-DLM, TRM88002 and TRM88003 represent three novel species of the genus , for which the names sp. nov. (type strain TRM66264-DLM=CCTCC AA 2021015=LMG 32389), sp. nov. (type strain TRM88002=CCTCC AA 2021036=LMG 32621) and sp. nov. (type strain TRM88003=CCTCC AA 2021037 =LMG 32622) are proposed.