Crotofolane Diterpenoids and Other Constituents Isolated from Croton kilwae .

Six new crotofolane diterpenoids (-) and 13 known compounds (-) were isolated from the MeOH-CHCl (1:1, v/v) extracts of the leaves and stem bark of . The structures of the new compounds were elucidated by extensive analysis of spectroscopic and mass spectrometric data. The structure of crotokilwaepoxide A () was confirmed by single-crystal X-ray diffraction, allowing for the determination of its absolute configuration. The crude extracts and the isolated compounds were investigated for antiviral activity against respiratory syncytial virus (RSV) and human rhinovirus type-2 (HRV-2) in HEp-2 and HeLa cells, respectively, for antibacterial activity against the Gram-positive and the Gram-negative , and for antimalarial activity against the Dd2 strain. -3β,19-Dihydroxykaur-16-ene () and ayanin () displayed anti-RSV activities with IC values of 10.2 and 6.1 μM, respectively, while exhibiting only modest cytotoxic effects on HEp-2 cells that resulted in selectivity indices of 4.9 and 16.4. Compounds and exhibited modest anti-HRV-2 activity (IC of 44.6 μM for both compounds), while compound inhibited HRV-2 with an IC value of 1.8 μM. Compounds - showed promising antiplasmodial activities (80-100% inhibition) at a 50 μM concentration.