Polymorphisms in SHISA3 and RFC3 genes and their association with feed conversion ratio in Hu sheep.
Frontiers in veterinary science(2022)
摘要
In animal husbandry, feed efficiency is a crucial economic trait. In this study, the general linear model was used to perform association analysis for various genotypes and feed conversion ratio (FCR)-related traits. Reverse transcription-quantitative PCR (RT-qPCR) was used to detect the expression of and mRNA levels in 10 tissues from 6 sheep. The results showed that SNPs in the NC_040257.1:c.625 T > C and NC_040261.1:g.9905 T > C were analyzed whether they were associated to feed efficiency parameters in Hu sheep (body weight, feed intake, average daily growth, and feed conversion ratio). NC_040257.1:c.625 T > C was shown to be significantly associated with body weight at 80, 100, and 120 days as well as feed conversion ratio ( < 0.05), whereas NC_040261.1:g.9905 T > C was found to be significantly associated with average daily weight gain from 80-140 days (ADG80-140) and FCR ( < 0.05). In Hu sheep, the CC genotypes of SHISA3 and RFC3 were the most common genotypes related to feed efficiency traits. Furthermore, the feed conversion ratio of the combined genotypes TT -CC , TT -CT , TT -TT , CT -CC and CT -CT was significantly better than the FCR of CC -TT . RT-qPCR results showed that the expression levels of were lower in the lung than in spleen, kidney, muscle and lymph ( < 0.05), and RFC3 was the lung had a highly significant higher expression level than the heart, liver, spleen, and muscle ( < 0.01). In conclusion, and polymorphisms can be used as genetic markers for improving feed conversion efficiency in Hu sheep.
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关键词
Hu sheep,RFC3,SHISA3,association analysis,feed conversion ratio,tissue expression
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