The complete chloroplast genome of Urtica angustifolia Fisch. ex Hornem. (Urticaceae), an important kind of traditional Chinese medicine in China.

Fisch. ex Hornem. is an important Chinese medicine. Here, the complete chloroplast genome of was assembled and characterized. The length of the chloroplast genome was 146,679 bp with the typical quadripartite structure, containing two inverted repeats (IRs) of 24,595 bp separated by a large single-copy (LSC) region of 79,820 bp and a small single-copy (SSC) region of 17,669 bp. The whole chloroplast genome of contains 111 genes, including 77 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Nucleotide variability analysis identified three hotspot regions (, , and ) for genomic divergence and 52 simple sequence repeats. Phylogenetic analysis based on the complete chloroplast genomes exhibited that formed a clade with and .