Characterization of the complete mitochondrial genome of Neolissochilus hendersoni (Herre, 1940) (Cypriniformes: Cyprinidae).

The differentiation between and species based only on morphology is ambiguous; however, phylogenetic analysis using their mitogenome sequences provides conclusive results. Here, the phylogenetic position of (Herre, 1940) was determined using its mitogenome data. Total DNA from was sequenced using the Illumina NovaSeq6000 platform, and annotation of mitochondrial genes was performed using MITOS2. Phylogenetic trees were constructed using the complete mitogenomes of 16 fish species. The mitogenome of was found to be 16584 bp long, containing two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes, and three non-coding control regions. The genome showed a slight A + T bias (A +  = 56.46%). Most PCGs were found to be located on the L-strand. Results of the phylogenetic analysis showed that is closely related to . Our results will help to clarify the phylogenetic relationship between and species.