Haematology, biochemistry and morphological features of peripheral blood cells in captive Boa constrictor

Abstract The common boa (Boa constrictor) belongs to the family Boidae and represents one of the most popular traded and kept snake species in captivity. The early diagnosis, prevention and prophylaxis of diseases in this species, and in reptiles in general, still pose major challenges, also due to the lack of reliable reference values. This prompted us to conduct a study on clinically healthy captive B. constrictor to assess their basic health parameters in the blood (haematological and biochemical values, stress markers). Several parameters differed significantly between younger (<3 years) and older (≥3 years) boas; in the latter, the percentages of eosinophils, the haemoglobin and haematocrit levels, as well as the albumin and total protein levels, were higher. In male snakes, cholesterol levels were significantly higher than in females. Light and electron microscopy as well as immunohistochemistry served to identify and determine the morphological features of peripheral blood cells, that is, heterophils, basophils, eosinophils, azurophils, monocytes, lymphocytes, thrombocytes and erythrocytes. Leukocyte subpopulations, that is, T and B cells and monocytes, were also identified based on specific marker expression. The study provides data on haematological, biochemical and stress hormone levels, suitable as reference values, and on the blood cell morphology of B. constrictor which can serve as a guideline for further research on this species.