Risk Factors for Exposure of Wild Birds to West Nile Virus in A Gradient of Wildlife-Livestock Interaction.

West Nile virus (WNV) transmission rate is shaped by the interaction between virus reservoirs and vectors, which may be maximized in farm environments. Based on this hypothesis, we screened for WNV in wild birds in three scenarios with decreasing gradient of interaction with horses: (i) the farm (A); (ii) the neighborhood (A); and (iii) a wild area (A). We captured wild birds and analyzed their sera for WNV antibodies by blocking ELISA and micro-virus neutralization test. infections were tested with generic and specific PCR protocols. We parameterized linear mixed models with predictors (bird abundance and diversity, vector abundance, vector host abundance, and weather quantities) to identify spp. and WNV exposure risk factors. We detected a low rate of infections by PCR (0.8%) and 6.9% of the birds were seropositive by ELISA. Exposure to spp. was higher in A (9%) than in A and A (5.6% and 5.8%, respectively). Bird diversity was the most relevant predictor of exposure risk and passerines dominated the on-farm bird community. Our results suggest that measures deterring the use of the farm by passerines should be implemented because the environmental favorability of continental Mediterranean environments for WNV is increasing and more outbreaks are expected.