A 3-dimensional molecular cartography of human cerebral organoids revealed by double-barcoded spatial transcriptomics
bioRxiv (Cold Spring Harbor Laboratory)(2023)
Abstract
Spatially-resolved transcriptomics is revolutionizing our understanding of complex tissues, but their current use for the exploration of a few sections is not representative of their 3-dimensional architecture. In this work we present a low-cost strategy for manufacturing molecularly double-barcoded DNA arrays, enabling large-scale spatially-resolved transcriptomics studies. We applied this technique to spatially resolve gene expression in several human brain organoids, including the reconstruction of a 3-dimensional view from multiple consecutive sections, revealing gene expression divergencies throughout the tissue.
### Competing Interest Statement
The authors have declared no competing interest.
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Key words
human cerebral organoids,spatial,double-barcoded
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