β-Alanine enhancing the crosslink of chitosan/poly-(γ-glutamic acid) hydrogel for a potential alkaline-adapted wound dressing

Tiny crosslink in chitosan (CS)/poly-(γ-glutamic acid) (γ-PGA) hydrogel leads to some disadvantages including low mechanical strength and high swelling. To enhance the crosslink of CS/γ-PGA hydrogel, amino acid (AA) was introduced to remove the drawbacks. The results indicated that AA can dramatically increase the crosslink and mechanical properties of CS/γ-PGA hydrogel, and AA chain length and concentration have a drastic effect on them. Particularly, 0.5 % β-Alanine (β-Ala) decreased the hydrogel by 70 % in porosity, 52 % in water solubility, and 30 % in swelling, but increased by 2.2-fold in elastic modulus, 2.08-fold in stress, and 1.53-fold in water retention. The porosity of the hydrogel correlates positively with the elastic modulus but negatively with the crosslinking degree. The effect of pH on CS/β-Ala/γ-PGA hydrogel was investigated in the load and release of benzalkonium chlorides (BAC). β-Ala strengthened pH response of the hydrogel in BAC load and release. The loading capacity increased with pH value, and 0.5 % β-Ala increased the hydrogel by 1.25-fold in the release capacity in alkaline environment, suggesting a good buffering effect of β-Ala on pH variation to accelerate the transportation of BAC. CS/β-Ala/γ-PGA hydrogel will be competently applied as a potential material for wound dressing in alkaline environment.