Comparison qPCR study for selecting a valid single copy gene for measuring absolute telomere length

Gene(2023)

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Abstract
•36B4 (RPLP0) is problematic to use in TL analyses.•An IFNB1 qPCR assay replaced 36B4 (RPLP0) but failed to show TL agreement when compared to DNAmTL results.•Analyses of the IFNB1 qPCR assay shows validated reasons for this lack of agreement.•Another IFNB1 qPCR assay avoids any discrepancies when using aTL analyses.
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Key words
Real time qPCR,aTL,TL,36B4 (RPLP0),Interferon B1 (IFNB1)
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