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链终止测序技术在RNA结构分析中的应用

WANG Deya, FAN Jiahui, TIAN Wei, ZHANG Ling, JIANG Yangfan,TIAN Zhongjing,DING Chengshi,KANG Meiling,WANG Yuhai

Laboratory Science（2022）

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Abstract
双脱氧链终止法是最常用的DNA测序技术,其核心是依赖双脱氧核糖核苷酸的扩增终止技术.在课堂授课中,教师对于双脱氧链终止法的原理及应用讲解较少,学生普遍对于该方法理解不足.基于以上问题,在核酸化学的教学内容上引入SHAPE(Selective 2'-Hydroxyl Acylation and Primer Extension)技术,不仅取得了较好的教学效果,同时增强了学生对于科研的兴趣.对SHAPE技术的原理及结果分析等教学设计简要叙述,以期为核酸化学的教学提供建议与参考.
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