Prevalence of Trypanosoma cruzi , the Etiologic Agent of Chagas Disease, Infection in Texas Skunks (Mammalia: Mephitidae).

Chagas disease is one of the world's most neglected tropical diseases, infecting over six million people across the Americas. The hemoparasite is the etiological agent for the disease, circulating in domestic, peridomestic, and sylvatic transmission cycles that are maintained by triatomine vectors and a diversity of wild and synanthropic hosts. Public health and wildlife management interventions targeting the interruption of transmission rely on an understanding of the dynamics driving the ecology of this zoonotic pathogen. One wildlife host that purportedly plays a role in the transmission of Chagas disease within the southern United States is the striped skunk (), although infection prevalence in this species is poorly understood. To this end, we conducted a PCR-based surveillance of in 235 wild skunks, representing 4 species, across 76 counties and 10 ecoregions in Texas, United States, along with an evaluation of risk factors associated with the infection. We recovered an overall prevalence of 17.9% for all mephitid taxa aggregated, ranging between 6.7% for plains spotted skunks () and 42.9% for western spotted skunks (). We report the first cases of infection in plains spotted and American hog-nosed skunks (), of important note for conservation medicine since populations of both species are declining within Texas. Although not statistically significant, we also detected trends for juveniles to exhibit greater infection risk than adults and for differential sex biases in prevalence between taxa, which align with variations in species-specific seasonal activity patterns. No geographic or taxonomic risk factors were identified. Our study contributed key data for population viability analyses and epidemiologic models in addition to providing a baseline for future surveillance among skunks and other wildlife species.