Actividad ovicida in vitro de la fracción rica en glicerol de Jatropha curcas L. en nematodos gastrointestinales de ovejas

Objective: To evaluate the effect exerted by the glycerol-rich fraction of Jatropha curcas L. oil, on the hatching of eggs from nematodes that parasitize sheep. Materials and Methods: A trial was conducted based on a complete randomized design. The inhibition of the hatching of eggs from sheep gastrointestinal nematodes in different concentrations of the glycerol-rich fraction (seriated dilutions from 100 to 156 mg/mL) was evaluated in vitro. Glycerin (10 v/v) was used as negative control, with analytical quality and distilled water. In addition, the components of the glycerol-rich fraction were identified through gas chromatography and the presence of phorbol esters was studied by mass spectrometry, coupled to a time-of-flight detector. Results: The glycerol-rich fraction recorded a strong ovicidal activity with dose-dependent effect, with mean lethal concentration of 3,60 × 10-4 mg/mL. No phorbol esters were found in the glycerol-rich fraction; while glycerin constituted the main compound (74,8 %). It was followed by palmitic, stearic, oleic and linoleic acids. Conclusions: The glycerol-rich fraction inhibits the hatching of eggs from parasite gastrointestinal nematodes in sheep, with dose-dependent effect. No phorbol esters were detected and the main component was glycerin.