CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing

Kyra L. Feuer,Marah H. Wahbeh,Christian Yovo,Eman Rabie,Anh-Thu N. Lam,Sara Abdollahi,Lindsay J. Young, Bailey Rike, Akul Umamageswaran,Dimitrios Avramopoulos

Scientific Reports（2022）

Cited 1|Views6

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Abstract

Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.

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