Protective effect of Perilla leaf extract against ROS formation and inflammation induced by TNF-α in A549 human lung carcinoma cell line
Songklanakarin Journal of Science and Technology (SJST)(2022)
Abstract
Perilla frutescens is an herb traditionally used to treat chronic diseases owing to its antioxidant and anti-inflammatory properties. This study aimed to determine the antioxidant and anti-inflammatory effects of perilla leaf extract (PLE) in TNF-α- treated A549 human lung carcinoma cell line. Perilla frutescens leaves were extracted with 70% ethanol, and the phenolic, flavonoid, and rosmarinic acid contents were determined. Cytotoxicity of PLE was assessed using the MTT assay. The antioxidant activity and intracellular ROS production in TNF-α-treated A549 cells were evaluated. Furthermore, mRNA expression of pro-inflammatory mediators was assessed by RT-qPCR. These results showed that PLE contained phenolic acid, flavonoids, and rosmarinic acid and had no cytotoxicity. PLE exhibits antioxidant activity through a reduction in intracellular ROS production in TNF-α-treated A549 cells. Moreover, PLE showed significantly decreased mRNA expression of IL-1β, IL-8, TNF-α and COX-2. Thus, PLE plays an important role as an antioxidant and anti-inflammatory agent for the prevention of ROS formation and inflammation in A549 cells.
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Key words
perilla frutescens,tnf-,antioxidant,anti-inflammatory,a549 cancer cells
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