Fe3O4 @polydopamine-based microchannel resistance immunosensor for detecting deoxynivalenol in wheat samples
Sensors and Actuators B: Chemical(2023)
Abstract
Food security problems caused by deoxynivalenol in grains have aroused widespread concern. Rapid analysis of deoxynivalenol still suffers from complex operation, low sensitivity, and insufficient accuracy. Herein, the Fe3O4 @polydopamine (PDA) nanoparticle with a core-shell structure was synthesized, which could conveniently conjugate the antibody of deoxynivalenol, thus forming a novel immunomagnetic separation carrier Fe3O4 @PDA-antibody for the specific enrichment of the target. Furthermore, the Fe3O4 @PDA-antibody nano-recognizer could act as the elicitor to activate the aggregation of complete antigen (BSA-deoxynivalenol) func-tionalized polystyrene (PS) beads through the antigen-antibody reaction. The aggregated Fe3O4 @PDA-antibody-antigen-PS immunocomplex was driven into the microchannel by the electroosmotic flow, inducing a remarkable change in current due to the blocking effect, which was related to the amount of deoxynivalenol. In this approach, Fe3O4 @PDA-antibody nanoparticles integrated extraction, immuno-reaction, and detection effec-tively, and the microchannel resistance sensor (MCRS) could immediately monitor the change in the current. The whole process could be completed within 1 h, which improved the efficiency and decreased the interference. This Fe3O4 @PDA-antibody-mediated MCRS was developed for the ultrasensitive detection of deoxynivalenol with a linear range of 0.05 -1000 ng/mL, and the limit of detection (LOD) was 20.7 pg/mL, which was a 21-fold enhancement compared with the enzyme-linked immunosorbent assay, providing a tool for hazardous target analysis.
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Key words
Blocking effect,Deoxynivalenol,Magnetic separation carrier,Microchannel resistance immunosensor
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