Wearable, nanofiber-based microfluidic systems with integrated electrochemical and colorimetric sensing arrays for multiplex sweat analysis

•We report a novel nanofiber-based microfluidic system for in situ sweat analysis.•Nanofibers are essential in sweat collection and reagent immobilization.•Electrochemical and colorimetric arrays were integrated in the system.•Simultaneous detection of glucose, lactate, pH, Cl− and urea was realized.•Key parts were made by simple techniques of electrospinning and direct writing.