薏苡叶斑病病原菌分离及其拮抗内生细菌的筛选

PU Feng-ya, HE Kai-xiao, YE Jian-ping, HE Qing-kang,GU Shu-jie,HE Yong-hong,YANG Zhi-qing

Acta Agriculturae Jiangxi（2022）

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Abstract

用组织块分离法和科赫式法则分离鉴定薏苡叶斑病病原菌,并用平板对峙法筛选抑制薏苡叶斑病病原菌生长的生防细菌.结果表明:分离得到的病原菌菌株Y2能够引起薏苡植株与田间病害一致的症状;通过ITS序列扩增,将Y2鉴定为弯孢霉(Curvularia coicis).通过平板对峙法筛选到1株对Y2有抑制作用的内生细菌菌株L47,L47对薏苡叶斑病病原菌的抑制率为73.82％;经过16S rDNA和gyrA序列分析,将L47鉴定为枯草芽孢杆菌(Bacillus subtilis).

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