Furfural removal from water by bioremediation process by indigenous Pseudomonas putida (OSBH3) and Pseudomonas aeruginosa (OSBH4) using novel suphala media: An optimization for field application

The release of furfural as effluent from industries has been considered a major pollution source since it adversely affects health and the environment. In a proper nutrient condition, the indigenous microbial removal process is always considered efficient, economical, and eco-friendly. Thus, the main objective is to employ the effective indigenous bacteria to remove the furfural from wastewater. In the present study, the indigenous isolates OSBH3 (strain number) and OSBH4 (strain number) were isolated from furfural-containing-oily-sludge and were identified by 16S-rRNA technique with >99% nucleotide similarity as Pseudomonas putida (P. putida) (Acc. No. GU329915) and Pseudomonas aeruginosa (P. aeruginosa) (Acc. No. GU329916) respectively. In a batch experiment, the furfural degradation was observed higher in the presence of the most efficient isolate P. putida (compare to P. aeruginosa) at different nutrient conditions including Suphala (Nitrogen-Phosphorous-fertilizer), glucose, and carbohydrate-free synthetic media (CFM). Generally, the furfural degradation rate was lower in CFM than the other substrates (Nitrogen-Phosphorous-fertilizer/glucose), in the presence of P. putida or P. aeruginosa. The furfural degradation efficiency was observed higher in presence of P. putida, compare to P. aeruginosa. Experimentally, the total (100%) degradation of furfural (500 mg/L) was noticed by P. Putida within 12h incubation time in presence of 1 mg/L Suphala. Even among different factors (based on ‘Taguchi L9 orthogonal array study’), the suphala (Nitrogen-Phosphorous-fertilizer) was influential in degrading the furfural at level 2, where the effects of pH and glucose were noticed at level 2 and level 2, respectively. The experimental findings supported the orthogonal array study positively. The bacterial (P. putida) growth rate and furfural degradation were promoted at the condition of 1 mg/L suphala, 2 mg/L glucose (pH 7.2), where the furfural was used by P. putida as the sole-carbon-source for growth. Thus, the indigenous P. putida (OSBH3; Acc. No. GU329915) (in presence of modified nitrogen-phosphorous-fertilizer media), can be applicable for furfural (Toxic) remediation from industrial effluent water by the eco-friendly process.