Fundidesulfovibrio agrisoli sp. nov., A Nitrogen-Fixing Bacterium Isolated from Rice Field

A strictly anaerobic nitrogen-fixing strain, designated SG106 T , was isolated from rice field. The 16S rRNA gene sequence analysis showed that strain SG106 T was closely related to the type strain of Fundidesulfovibrio magnetotacticus (97.3%). In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic (constructed using a concatenated alignment of 117 conserved bacterial single-copy genes with GTDB-Tk) trees, strain SG106 T clustered with members of the genus Fundidesulfovibrio . Strain SG106 T grew at 20–40 °C and 0–0.4% (w/v) NaCl. Desulfoviridin was found in the strain SG106 T . The genomic DNA G + C content of strain SG106 T was 66.0%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SG106 T and the closely related F. magnetotacticus were 78.4% and 21.7%, respectively. Genome analysis showed that strain SG106 T encodes genes for nitrogen fixation ( nifHDK ). Acetylene reduction experiments showed that the nitrogenase activity of strain SG106 T could reach 224.7 μmol C 2 H 4 g −1 protein h −1 . Based on the above results, strain SG106 T represents a novel species of the genus Fundidesulfovibrio , for which the name Fundidesulfovibrio agrisoli sp. nov. is proposed. The type strain is SG106 T (= GDMCC 1.3136 T = JCM 35588 T ).