A CELL THERAPY ASSISTED NOVEL MICROFLUIDIC DEVICE PROMOTES IN VITRO SPERMATOGENESIS IN NEONATAL MICE

Chemo-radiotherapy applications result in permanent infertility in half of male pediatric cancer survivors1. Spermatogonial stem cells (SSC) constitute the only option for fertility preservation since spermatogenesis is not initiated yet2. The rationale of study is that bone marrow derived mesenchymal stem cells (BMMSC) have similar embryonic origin and gene expression profile with Sertoli cells3,4 that play crucial role in proliferation of SSCs, and microfluidic devices (MD) simulate microvascular flow in the body that could promote for in vitro spermatogenesis (IVS). We hypothesized that, syngeneic BMMSC-conditioned medium (BMMSC-CM) contributed monolayer pumpless MD leads to survival, expansion and differentiation of SSC pool in vitro.