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A RNAi-based strategy to suppress Micropterus salmoides rhabdovirus in GCO cells and largemouth bass by silencing envelope glycoprotein

Xuemei Yuan,Jiayun Yao,Lei Huang,Lingyun Lin,Xiaoyi Pan,Jinbiao Jiao,Haiqi Zhang

Aquaculture（2023）

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Abstract
•Identification of an siRNA sequence for effective knockdown of MSRV G protein.•Construction of an shRNA plasmid for MSRV G protein knockdown in CHO cells and largemouth bass larvae.•G protein knockdown alleviates cytotoxic effect of MSRV infection and promotes the survival of largemouth bass larvae.
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Key words
Largemouth bass,Rhabdovirus,RNA interference,shRNA
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