Composition Characterization and Biological Activity Study of Achillea vermicularis Extract and Extract Loading Nanoparticles.

Antiproliferative activity of Achillea vermicularis extracts were calculated on glial (C6) and keratinocyte (HaCaT) cell lines using XTT assay. It was observed that all extracts of Achillea vermicularis at the determined concentration were not cytotoxic activity in HaCaT cell lines. The NPs of the extract with the best cytotoxic activity was prepared and necessary characterization studies were performed. Results showed that NP containing the extract has a lower IC value and more cytotoxic activity in C6 cells compared to the only extract. Furthermore, the antiepileptic potentials of these substances were explored in this study. The effect of Achillea vermicularis extracts on the enzyme activities of carbonic anhydrase I and II isoenzymes (hCA I and hCA II) was measured using spectrophotometry to achieve this goal. Achillea vermicularis extracts demonstrated high inhibitory activities compared to standard inhibitor (Acetazolamide, AAZ) with IC values in the range of 5.04-10.8 μg/mL for hCA I, 5.40-9.22 μg/mL for hCA II. HPLC-DAD was used in this investigation to assess the main chemicals found in the extract and nanoparticle. The results showed that the ethanol extract (157.636 μg/mg extract) and nanoparticle (4.631 μg/mg extract) had a significant amount of the 8-hydroxysalvigenin component. This article is protected by copyright. All rights reserved.