Impact of heat-inactivated Lactobacillus on inflammatory response in endotoxin- and chemotherapeutic-treated porcine enterocytes

Several factors such as pathogen bacteria, or oral chemotherapy disturb the intestinal integrity, leading to several undesirable effects. Inactivated probiotics may be beneficial in safely redress the physiological functions of the intestinal epithelium.Our aim is to determine the effect of tyndallized Lactobacillus on LPS-and 5-fluorouracil-treated porcine je-junal cells.IPEC-J2 cells derived from porcine jejunal epithelium were used as the in vitro model. The enterocyte cell cultures were treated with 109 Lactobacillus reuteri cells/ml or 10 mu g/ml lipopolysaccharides (LPS) or 100 mu M 5-fluorouracil separately and simultaneously. We determined the alterations in mRNA levels of inflammatory mediators IL6, CXCL8/IL8, TNF. Furthermore, the protein level of IL-6 and IL-8 were measured.The fluorouracil treatment upregulated the IL6 gene expression, the endotoxin treatment upregulated the IL8 and TNF level. The heat-inactivated Lactobacillus increased the IL-8 level both at the gene expression and protein level. The co-administration of the non-viable probiotic with the 5-fluorouracil and the LPS resulted in decrease of IL6, IL8, and TNF level.The immune-modulator effect of tyndallized probiotic product is demonstrated in porcine jejunal cells. The inactivated Lactobacillus was able to prevent the accumulation of the selected inflammatory mediators in LPS-or 5-fluorouracil-exposed enterocytes.