Evaluating drug efficacy by visualizing the spatiotemporal dynamics of intracellular states using a novel Covariation Network analysis

To capture the spatiotemporal dynamics of biomolecules for examining the pharmacological action of drugs, we developed a novel protein network analysis, Protein Localization and Modification-based Covariation Network (PLOM-CON) analysis method. This method quantifies the temporal changes in quantity, quality (post-translational modifications such as phosphorylation), and (co)localization of proteins that are activated or inactivated in response to specific signals input from the immunofluorescence images, and visualizes them as a "covariation network" using the "strength of temporal correlation" of the feature quantities.