Optimized study of an in vitro 3D culture of preantral follicles in mice.

Hehe Ren, Yingxin Zhang, Yanping Zhang, Yikai Qiu,Qing Chang,Xiaoli Yu,Xiuying Pei

Journal of veterinary science（2023）

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摘要

The present study furnishes insights into the mature oocytes obtained from the 3D culture of the preantral follicle by using ultra-low attachment 96-well plate with an FBS-free system and supports the clinical practices to achieve competent, mature oocytes for fertilization.

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关键词

3D culture,Preantral follicles,sodium alginate,ultra-low attachment plate

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