Cloning, Characterization and Expression Analysis of Caspase-8 Genes from the Golden Pompano (trachinotus Ovatus)
FRONTIERS IN MARINE SCIENCE(2022)
Abstract
Bacterial infection triggers apoptosis and immunity by regulating the expression of caspases. In this article, we report two caspase-8 paralogs, termed ToCas8 and ToCas8-like, from an economically important teleost golden pompano (Trachinotus ovatus). Caspase-8 is a classic cysteine protease that is essential for the initiation and execution of apoptosis and is a critical regulator in initiating death receptor-induced programmed cell death. The two proteins encoded by ToCas8 and ToCas8-like were characterized by multiple sequence alignment and protein structure analysis showing similarity to their counterparts in vertebrates and exhibiting typical features of the Caspase-8 protein family. Gene synteny analysis further confirmed that two isoforms of caspase-8 genes exactly existed in golden pompano. Phylogenetic analysis showed that the Caspase-8 proteins of the selected species in the study were clustered together, and Cas8 and Cas8-like were clustered into two different subgroups. In addition, ToCas8 and ToCas8-like were widely distributed and both were expressed at high levels in gill, kidney and intestinal tissues. Transcript levels of ToCas8 and ToCas8-like were significantly upregulated in response to LPS and Poly(I:C) challenges, suggesting that caspase-8 genes are involved in the immune response to pathogens. In summary, we have identified two isoforms of caspase-8 in golden pompano that are involved in the immune response. These findings will expand our understanding of the evolutionary history and functional characteristics of caspase-8 genes in vertebrates.
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Key words
Trachinotus ovatus,caspase-8,phylogenetic analysis,tissue distribution,LPS challenge,Poly(I:C) stimulation
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