Canibacter zhuwentaonis sp. nov. and Canibacter zhoujuaniae sp. nov. , isolated from Marmota himalayana .

Four Gram-stain-positive, facultatively anaerobic, non-motile, non-spore-forming and rod-shaped bacteria (lx-72, lx-45, ZJ784 and ZJ955) were isolated from the respiratory tract or faeces of marmot () from the Qinghai-Tibet Plateau in China. Analysis of the 16S rRNA gene sequences showed that all strains belong to the genus and are more related to CCUG 64069 (95.1-97.4 % similarity) than to the genus . Both strain pairs grew well at pH 6-9 and 15-42°C, and ZJ784/ZJ955 could tolerate slightly higher NaCl (0.5-4.5 %, w/v) than lx-72/lx-45(0.5-3.5 %). Based on whole-genome sequences, the average nucleotide identity and digital DNA-DNA hybridization values between our four isolates and their closest relative were below the species delineation thresholds of 70 % and 95-96 %. The common major fatty acids (>10 %) of our four strains were anteiso-C and anteiso-C. For both new type strains, MK-8(H) and MK-9(H) were the major isoprenoid quinones, and diphosphatidylglycerol and phosphatidylglycerol were the main polar lipids. The genomic DNA G+C content of all strains was 53.9 mol%. Based on results from the genomic comparison, phylogenetic analysis, and physiological and biochemical characteristics, the four isolates represent two novel species in the genus , for which the names sp. nov. (type strain lx-72=KCTC 49658=GDMCC 1.2569) and sp. nov. (type strain ZJ784=KCTC 49507=GDMCC 1.1997) are proposed.